Phospho-PLK Binding Motif (ST*P) (D73F6) Rabbit mAb #5243
- WB
- IP
Supporting Data
REACTIVITY | All |
SENSITIVITY | Endogenous |
MW (kDa) | |
Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- All-All Species Expected
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:100 |
Peptide ELISA (DELFIA) | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Phospho-PLK Binding Motif (ST*P) (D73F6) Rabbit mAb detects endogenous levels of cellular proteins containing the Ser-phospho-Thr-Pro motif. This antibody has minor cross-reactivity with phospho-Thr-Pro motifs.
Species Reactivity:
All Species Expected
Source / Purification
Monoclonal antibody is produced by immunizing animals with an SpTP peptide library.
Background
Polo-like kinases (PLKs) are Ser/Thr protein kinases that play an essential role during the cell cycle. At least four PLKs exist in mammalian cells: PLK1, PLK2, PLK3, and PLK4 (1). PLKs have a highly conserved amino-terminal kinase domain and a relatively divergent carboxy-terminal domain called the Polo-box domain (PBD). Of the four PLKs, PLK1 is the best characterized (2). PLK1 functions as a key regulator of mitotic events by phosphorylating substrate proteins on centrosomes, kinetochores, the mitotic spindle, and the midbody, and is crucial for proper progression through multiple stages of mitosis (2-4). The PBDs of PLK1 function as a phospho-Ser/Thr-binding module, recognizing the optimal recognition sequence motif Ser-(pSer/pThr)-(Pro/X) (5). Binding of phosphopeptides containing the Ser-(pSer/pThr)-(Pro/X) motif by the PBD in PLK1 relieves its inhibitory function on kinase activity (6). Ser-(pSer/pThr)-Pro peptides are phosphorylated by proline-directed kinases such as cyclin dependent kinases (CDKs). These findings imply that priming phosphorylations on substrates or docking proteins by other mitotic kinases such as CDKs may target PLK1 to its substrates and simultaneously activate its kinase activity. Phospho-PLK Binding Motif (ST*P) (D73F6) Rabbit mAb is a useful tool to study PLK1 binding proteins and PLK1 substrates.
Limited Uses
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