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Render Timestamp:
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4/5/2025, 1:06:39 AM UTC
Commit: c91f970ca8df4f527662a05c7bd6e4d03c6fa173
XML generation date: 2025-03-10 22:05:07.316
Product last modified at: 2025-04-01T08:00:21.679Z
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PDP - Template Name: Monoclonal Antibody (Isotype Control)
PDP - Template ID: *******4b30f73
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Mouse (E7Q5L) mAb IgG2b Isotype Control #53484

Filter:
  • IP
  • IHC
  • IF
  • F
  • ChIP
Immunoprecipitation Image 1: Mouse (E7Q5L) mAb IgG2b Isotype Control
Immunoprecipitation of IDH2 from Jurkat cell extracts. Lane 1 is 10% input, lane 2 is Mouse (E7Q5L) mAb IgG2b Isotype Control, and lane 3 is IDH2 (KrMab-3) Mouse mAb #60322. Western blot analysis was performed using IDH2 (D8E3B) Rabbit mAb #56439. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as the secondary antibody.

To Purchase # 53484

Cat. # Size Qty. Price
53484S 100 µl
$149

Supporting Data

Concentration 2.5 mg/ml
SENSITIVITY Endogenous
Source/Isotype Mouse IgG2b
Application Key:
  • IP-Immunoprecipitation 
  • IHC-Immunohistochemistry 
  • IF-Immunofluorescence 
  • F-Flow Cytometry 
  • ChIP-Chromatin Immunoprecipitation 
  • Related Products
  • Conjugates

Product Information

Product Usage Information

Important! This control antibody must be diluted to the same concentration (not dilution) as the specific antibody used in analysis. Higher background fluorescence may result if excessive amounts of isotype control are used. For protocol details, please reference the product page for the specific antibody used in analysis.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Mouse (E7Q5L) mAb IgG2b Isotype Control is not directed against any known antigen. It functions as an isotype control for mouse IgG2b monoclonal antibodies.

Background

Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
    For Research Use Only. Not For Use In Diagnostic Procedures.
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