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Commit: c91f970ca8df4f527662a05c7bd6e4d03c6fa173
XML generation date: 2025-03-10 22:04:07.723
Product last modified at: 2025-04-02T22:30:15.896Z
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PDP - Template Name: Monoclonal Antibody (Isotype Control)
PDP - Template ID: *******4b30f73
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Mouse (G3A1) mAb IgG1 Isotype Control #5415

Filter:
  • IP
  • IHC
  • IF
  • F
  • ChIP
Immunoprecipitation Image 1: Mouse (G3A1) mAb IgG1 Isotype Control
Immunoprecipitation of cleaved PARP (Asp214) from serum-starved HeLa cells treated with Staurosporine #9953 (1 μM, 3 hr). Lane 1 is 10% input, lane 2 is Mouse (G3A1) mAb IgG1 Isotype Control, and lane 3 is Cleaved PARP (Asp214) (E2T4K) Mouse mAb #32563. Western blot was performed using Cleaved PARP (Asp214) (E2T4K) Mouse mAb #32563. Anti-mouse IgG, HRP-linked Antibody #7076 was used as a secondary antibody.

To Purchase # 5415

Cat. # Size Qty. Price
5415S 250 µg
$149

Supporting Data

Concentration 2.5 mg/ml
SENSITIVITY Endogenous
Source/Isotype Mouse IgG1 kappa
Application Key:
  • IP-Immunoprecipitation 
  • IHC-Immunohistochemistry 
  • IF-Immunofluorescence 
  • F-Flow Cytometry 
  • ChIP-Chromatin Immunoprecipitation 
  • Related Products
  • Conjugates

Product Information

Product Usage Information

Important! This control antibody must be diluted to the same concentration (not dilution) as the specific antibody used in analysis. Higher background fluorescence may result if excessive amounts of isotype control are used. For protocol details, please reference the product page for the specific antibody used in analysis.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #87582.

Specificity / Sensitivity

Mouse (G3A1) mAb IgG1 Isotype Control is not directed against any known antigen. It functions as an isotype control for mouse IgG1 monoclonal antibodies.

Background

Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
    For Research Use Only. Not For Use In Diagnostic Procedures.
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