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Androgen Receptor Antibody (Carboxy-terminal Antigen)

Androgen Receptor Antibody (Carboxy-terminal Antigen) #54653

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Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	110
SOURCE	Rabbit

Application Key:

WB-Western Blotting
IP-Immunoprecipitation

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Androgen Receptor Antibody (Carboxy-terminal Antigen) recognizes endogenous levels of total Androgen Receptor protein. This antibody does not detect AR-V7 isoform.

Species Reactivity:

Human

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val662 of human Androgen Receptor protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Androgen receptor (AR), a zinc finger transcription factor belonging to the nuclear receptor superfamily, is activated by phosphorylation and dimerization upon ligand binding (1). This promotes nuclear localization and binding of AR to androgen response elements in androgen target genes. Research studies have shown that AR plays a crucial role in several stages of male development and the progression of prostate cancer (2,3).

Database Links

UniProt ID: P10275

Entrez-Gene Id: 367

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For Research Use Only. Not For Use In Diagnostic Procedures.

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