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Product last modified at: 2025-03-08T08:04:58.179Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

CD93/C1qR (E5H9B) Rabbit mAb (BSA and Azide Free) #55570

Filter:
  • WB
  • IHC
  • F
Western Blotting Image 1: CD93/C1qR (E5H9B) Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from various cell lines using CD93/C1qR (E5H9B) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Negative expression of CD93/C1qR protein in GRANTA 519 and Raji cells is consistent with the predicted expression pattern. Data were generated using the standard formulation of this product.

To Purchase # 55570

Cat. # Size Qty. Price
55570SF 100 µg
$843

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 120, 82
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IHC-Immunohistochemistry 
  • F-Flow Cytometry 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Usage Information

This product is the carrier free version of product #55011. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

Formulation

Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

For standard formulation of this product see product #55011

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

CD93/C1qR (E5H9B) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total CD93/C1qR protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of human CD93/C1qR protein.

Background

CD93, also known as complement component C1q receptor (C1qR), is a type-I transmembrane glycoprotein belonging to the CLEC14 family of surface antigens, which share a similar ectodomain architecture highlighted by the presence of a C-type lectin domain and multiple EGF-like domains. CD93/C1qR expression is primarily restricted to endothelial cells associated with normal and tumor-associated vasculature as well as immune cells of the myeloid lineage, including monocytes and neutrophils (1). The extracellular domain of CD93/C1qR is rich in O-linked glycosylation modifications, which stabilizes its surface expression by protecting it from proteolytic cleavage (2,3). CD93/C1qR regulates various aspects of endothelial cell biology, including proliferation, migration, and adhesion (4). Indeed, research studies have demonstrated that surface-expressed CD93/C1qR binds to an endothelial-specific extracellular matrix ligand, multimerin-2, and disrupting the interaction compromises angiogenesis (5). Furthermore, CD93 expression is upregulated in tumor vasculature and correlates with poor survival (6-8).
For Research Use Only. Not For Use In Diagnostic Procedures.
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