HOXD9 (E3F2T) Rabbit mAb #55962
- WB
- IP
- ChIP
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 42 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Usage Information
For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 × 10^6 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Chromatin IP | 1:50 |
Storage
Protocol
Specificity / Sensitivity
HOXD9 (E3F2T) Rabbit mAb recognizes endogenous levels of total HOXD9 protein. This antibody does not cross-react with HOXA9, HOXB9 or HOXC9 proteins. This antibody also weakly recognizes non-specific bands of unknown origin at 55 and 70 kDa in some cell lines.
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly159 of human HOXD9 protein.
Background
Homeobox protein Hox-D9 (HOXD9) is a sequence-specific transcription factor that is part of a developmental regulatory program that provides cells with specific positional identities on the anterior-posterior axis. HOXD9 is developmentally expressed in structures of either mesodermal or neuro-ectodermal origin, such as developing limbs, gonads, and the central nervous system (1-6). HOXD9 plays a critical role in regulation of limb development, neuronal development, and development of mammary glands and gonads in many organisms (1-6). The HOXD9 gene promoter is found to be hypermethylated and silenced in multiple types of cancer, including breast cancer, melanoma brain metastases, and cholangiocarcinomas (7-9). In addition, HOXD9 expression is increased in other types of cancer, including human glioblastomas and astrocytomas, where expression appears to drive growth of the tumors (10).
Limited Uses
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