Render Target: STATIC
Render Timestamp: 2024-11-21T13:28:23.026Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-04-24 09:11:02.470
Product last modified at: 2024-05-30T07:12:12.842Z
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PDP - Template Name: Secondary Antibody
PDP - Template ID: *******76815a7

Goat Anti-Mouse IgG2a Antibody (AP Conjugate) #61906

Filter:
  • WB

    Supporting Data

    REACTIVITY M
    SENSITIVITY
    MW (kDa)
    Source/Isotype Goat
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Description

    Affinity purified Goat Anti-Mouse IgG2a antibody is conjugated to alkaline phosphatase (AP). This product has been optimized for use as a secondary antibody in Western blotting applications.

    Product Usage Information

    Recommended Antibody Dilutions:

    1:2000 - 1:8000

    Recommended antibody dilutions are provided in ranges because the optimal dilution is dependent on many factors, such as antigen density and permeability.

    Storage

    Supplied in 50 mM Tris, 1 mM MgCl2, pH 8.0, and 50% glycerol containing < 0.1% sodium azide. Store at -20°C. Do not aliquot the antibody.

    Specificity / Sensitivity

    Goat Anti-Mouse IgG2a Antibody (AP Conjugate) detects the heavy chain of mouse IgG2a but does not cross-react with other mouse IgG subclasses, mouse IgM, or mouse IgA. This antibody has minimal cross-reaction with human, bovine, and horse serum proteins. It may cross-react with immunoglobulins from other species.

    Species Reactivity:

    Mouse

    Source / Purification

    Goat Anti-Mouse IgG2a Antibody (AP Conjugate) is produced by immunizing goats with mouse IgG2a. The Anti-Mouse IgG2a antibody is purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

    Background

    The alkaline phosphatase (AP) conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent or other substrates for detection on Western blots. One of the advantages of AP conjugation is that the reaction rate remains linear for a long period of time.
      For Research Use Only. Not For Use In Diagnostic Procedures.
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