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PDP - Template Name: Flow Cytometry Panels
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  1. Products /
  2. Flow Cytometry Kits & Reagents /
  3. Mouse Activated T Cell Markers Flow Cytometry Panel

Mouse Activated T Cell Markers Flow Cytometry Panel #62447

    Product Information

    Protocol

    Product Description

    The Mouse Activated T Cell Markers Flow Cytometry Panel can be used to identify activated mouse T cells.

    T cells are identified by expression of CD3. There are two major subsets of conventional T cells: helper T cells which express CD4, and cytotoxic T cells which express CD8. Upon stimulation through the T cell receptor, T cells upregulate expression of CD69 and IL-2Rα/CD25. Regulatory T cells constitutively express IL-2Rα/CD25.

    Product Usage Information

    All antibodies in this kit are compatible with the Flow Cytometry, Live Cell Protocol for Directly Conjugated Antibodies and can be used in a single staining mix. After antibody staining and prior to acquisition on a flow cytometer, we recommend adding a membrane impermeable viability dye such as Propidium Iodide or 7-AAD to enable identification and exclusion of dead cells from the analysis.

    Gating strategy for identifying activated T cells:
    If Propidium Iodide or 7-AAD was used, first gate on viable cells. Next, gate on lymphocytes based on forward scatter and side scatter. Helper T cells are the CD3+CD4+ cells within the lymphocyte gate. Activated helper T cells are positive for expression of CD69 and/or IL-2Rα/CD25. Regulatory T cells can also express IL-2Rα/CD25. Cytotoxic T cells are the CD3+CD8+ cells within the lymphocyte gate. Activated cytotoxic T cells are positive for expression of CD69 and/or IL-2Rα/CD25.

    Storage

    Antibodies are supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2. Store at 4oC. Do not aliquot the antibodies. Protect from light. Do not freeze.

    All components in this kit are stable in accordance with the date printed on the outer packaging label when stored at the recommended temperature. Please refer to product labels, datasheets, or web pages for specific “Best By” dates for each individual component.

    Specificity / Sensitivity

    Each antibody in the Mouse Activated T Cell Markers Flow Cytometry Panel detects endogenous levels of its target protein. CD3 (17A2) Rat mAb (PE Conjugate) recognizes CD3ε, CD3γ, and CD3δ proteins and detects epitopes within the extracellular domains. CD4 (RM4-5) Rat mAb (violetFluor™ 450 Conjugate), CD8α (2.43) Rat mAb (PE-Cy7® Conjugate), IL2-Rα/CD25 (PC61.5) Rat mAb (PerCP-Cy5.5® Conjugate), and CD69 (H1.2F3) Hamster mAb (FITC Conjugate) detect epitopes within the extracellular domains.

    Species Reactivity:

    Mouse

    Source / Purification

    Monoclonal antibodies were purified from tissue culture supernatant via affinity chromatography. The purified antibodies were conjugated under optimal conditions, with unreacted dye removed from the preparation.

    Limited Uses

    Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
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