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IFN-γ (XMG1.2) Rat mAb (PE Conjugate)

IFN-γ (XMG1.2) Rat mAb (PE Conjugate) #62675

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Supporting Data

REACTIVITY	M
SENSITIVITY	Endogenous
MW (kDa)
Source/Isotype	Rat IgG1 kappa

Application Key:

F-Flow Cytometry

Species Cross-Reactivity Key:

M-Mouse

Product Information

Product Description

This Cell Signaling Technology antibody is conjugated to PE and tested in-house for direct flow cytometric analysis in mouse cells.

Product Usage Information

For optimal flow cytometry results, we recommend 0.125 μg of antibody per test.

Application	Dilution
Flow Cytometry (Fixed/Permeabilized)	1:160

Storage

Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.

Protocol

Specificity / Sensitivity

IFN-γ (XMG1.2) Rat mAb (PE Conjugate) recognizes endogenous levels of total IFN-γ protein. This antibody detects an epitope within the intracellular domain.

Species Reactivity:

Mouse

Source / Purification

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Background

IFN-γ plays key roles in both the innate and adaptive immune response. IFN-γ activates the cytotoxic activity of innate immune cells, such as macrophages and NK cells (1,2). IFN-γ production by NK cells and antigen presenting cells (APCs) promotes cell-mediated adaptive immunity by inducing IFN-γ production by T lymphocytes, increasing class I and class II MHC expression, and enhancing peptide antigen presentation (1). Due to differences in the degree of glycosylation, there are three forms of IFN-γ, with approximate molecular weights of 25, 20, and 15.5 kDa by SDS-PAGE (5). The anti-viral activity of IFN-γ is due to its induction of PKR and other regulatory proteins. Binding of IFN-γ to the IFNGR1/IFNGR2 complex promotes dimerization of the receptor complexes to form the (IFNGR1/IFNGR2)₂ -IFN-γ dimer. Binding induces a conformational change in receptor intracellular domains and signaling involves Jak1, Jak2, and Stat1 (3). The critical role of IFN-γ in amplification of immune surveillance and function is supported by increased susceptibility to pathogen infection by IFN-γ or IFNGR knockout mice and in humans with inactivating mutations in IFNGR1 or IFNGR2. IFN-γ also appears to have a role in atherosclerosis (4).

Database Links

UniProt ID: P01580

Entrez-Gene Id: 15978

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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