Render Target: STATIC
Render Timestamp: 2025-01-21T04:24:35.170Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-05-10 22:35:03.491
Product last modified at: 2025-01-01T09:04:09.292Z
Cell Signaling Technology Logo

Basket Updated

0

Items added

1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

DNAM-1/CD226 (E8L9G) XP® Rabbit mAb #66631

Filter:
  • WB
  • IHC
Western Blotting Image 1: DNAM-1/CD226 (E8L9G) XP® Rabbit mAb
Western blot analysis of extracts from various cell lines using DNAM-1/CD226 (E8L9G) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

To Purchase # 66631

Cat. # Size Qty. Price Ships
66631T 20 µl
$199
66631S 100 µl
$463

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 70
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IHC-Immunohistochemistry 
Species Cross-Reactivity Key:
  • H-Human 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
IHC Leica Bond 1:50 - 1:200
Immunohistochemistry (Paraffin) 1:50 - 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #66991.

Protocol

Specificity / Sensitivity

DNAM-1/CD226 (E8L9G) XP® Rabbit mAb recognizes endogenous levels of total DNAM-1/CD226 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human DNAM-1/CD226 protein.

Background

DNAM-1/CD226 is a member of the immunoglobulin-like superfamily. It is expressed on T cells, natural killer (NK) cells, monocytes, and macrophages. It is a major activating receptor on NK cells. Engagement with its ligands Nectin-2 (CD112) and PVR (poliovirus receptor, also known as CD155) on the target cells leads to downstream activation of ERK, AKT, and PLCg2 signaling pathways that are crucial for NK activation and NK-mediated killing (1-4). LFA-1 physically associates with DNAM-1/CD226 and contributes to its signaling (5). Activating signal of DNAM-1/CD226 is counterbalanced by inhibitory receptors TIGIT and CD96, competing for the common ligands CD122 and PVR (6,7).
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStain is a registered trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.