CD54/ICAM-1 (E3Q9N) XP® Rabbit mAb #67836
- WB
- IHC
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 89, 92 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| IHC Leica Bond | 1:100 - 1:400 |
| Immunohistochemistry (Paraffin) | 1:100 - 1:400 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier-free (BSA and azide free) version of this product see product #26104.
Protocol
Specificity / Sensitivity
CD54/ICAM-1 (E3Q9N) XP® Rabbit mAb recognizes endogenous levels of total CD54/ICAM-1 protein.
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro410 of human CD54/ICAM-1 protein.
Background
Intercellular cell adhesion molecule-1 (CD54 or ICAM-1) is a cell surface glycoprotein that belongs to the immunoglobulin superfamily (IgSF) of adhesion molecules. CD54 is expressed at low levels in diverse cell types, and is induced by cytokines (TNF-α, interleukin-1) and bacterial lipopolysaccharide (1). Apical localization of CD54 on endothelial cells (or basolateral localization on epithelial cells) is a prerequisite for leukocyte trafficking through the endothelial (or epithelial) barrier (1). Apical expression of CD54 on epithelial cells mediates pathogen invasion as well as host defense, a pattern also observed in tumors (1). CD54 also functions as a co-stimulator on antigen presenting cells, binding to its receptor LFA-1 (leukocyte function-associated antigen-1) on the surface of T cells during antigen presentation (2). Cross-linking of CD54 or binding to its ligand triggers activation of Src family kinases and the Rho/ROCK pathway (3-7). Phosphorylation on Tyr485 of CD54 is required for its association with SHP-2 (5). SHP-2 seems essential for CD54-induced Src activation (7).
- Hopkins, A.M. et al. (2004) Adv Drug Deliv Rev 56, 763-78.
- Nishibori, M. et al. (2003) J Pharmacol Sci 92, 7-12.
- Holland, J. and Owens, T. (1997) J Biol Chem 272, 9108-12.
- Etienne, S. et al. (1998) J Immunol 161, 5755-61.
- Pluskota, E. et al. (2000) J Biol Chem 275, 30029-36.
- Muro, S. et al. (2003) J Cell Sci 116, 1599-609.
- Wang, Q. et al. (2003) J Biol Chem 278, 47731-43.
Limited Uses
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