Render Target: STATIC
Render Timestamp: 2024-12-20T10:55:08.876Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-08-01 15:26:30.259
Product last modified at: 2024-12-10T15:00:15.416Z
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PDP - Template Name: Polyclonal Antibody (Epitope Tag)
PDP - Template ID: *******04464df

HPC4-Tag Antibody #68083

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY All
    SENSITIVITY Transfected Only
    MW (kDa)
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • All-All Species Expected 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    HPC4-Tag Antibody recognizes recombinant proteins containing the HPC4-tag. This antibody recognizes the HPC4-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells.

    Species Reactivity:

    All Species Expected

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues of the HPC4-tag.

    Background

    Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.

    HPC4-tag is a 12 amino acid sequence derived from protein C, which is a vitamin K-dependent plasma zymogen. Protein C is activated by proteolytic cleavage of the thrombin-thrombomodulin complex to form an anticoagulant enzyme (1,2). This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion (3,4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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