SignalFire™ ECL Reagent #6883
To Purchase # 6883
| Cat. # | Size | Qty. | Price |
|---|---|---|---|
| 6883P3 | 50 ml 25 ml each substrate | $45 | |
| 6883S | 500 ml 250 ml each substrate | $344 |
- Product Includes
- Related Products
Kit Includes | Petite Kit (P) Quantity | Small Kit (S) Quantity |
|---|---|---|
SignalFire™ ECL Reagent A | 1 x 25 ml | 1 x 250 ml |
SignalFire™ ECL Reagent B | 1 x 25 ml | 1 x 250 ml |
Product Information
Product Usage Information
1. Wash membrane-bound HRP (antibody conjugate) three times for 5 minutes in TBS/T: (20 mM Tris-HCl (pH 7.6),137 mM NaCl and 0.1% Tween-20). It is very important to thoroughly wash the membrane prior to substrate incubation.
2. Prepare 1x SignalFire™ Plus ECL Reagent by diluting one part 2X Reagent A (#65293) and one part 2X Reagent B (#82201) (e.g., for 10 ml, add 5 ml Reagent A and 5 ml Reagent B). Mix well.
3. Incubate substrate with membrane for 1 minute, remove excess solution (membrane remains wet), wrap in plastic, and expose to X-ray film.
*Avoid repeated exposure to skin (see enclosed Material Safety Data Sheet or refer to our website for further information).
Storage
Product Description
Background
Alternate Names
Chemiluminescence; ECL; HRP; Luminol; Signal Fire; Substrate
Limited Uses
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