Render Target: STATIC
Render Timestamp: 2024-11-22T11:37:39.358Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-10-22 16:01:07.167
Product last modified at: 2024-11-18T15:00:09.128Z
View Featured Offers >>
1% for the planet logo
PDP - Template Name: Detection System Kit
PDP - Template ID: *******2b9e861
  1. Products /
  2. WB & IP Reagents /
  3. SignalFire™ ECL Reagent

SignalFire™ ECL Reagent #6883

    Product Information

    Product Usage Information

    (a) Wash membrane-bound HRP (antibody conjugate) three times for 5 minutes in TBS/T.
    (b) Prepare 1x SignalFire™ Plus ECL Reagent by diluting one part 2X Reagent A (#65293) and one part 2X Reagent B (#82201) (e.g., for 10 ml, add 5 ml Reagent A and 5 ml Reagent B). Mix well.
    (c) Incubate substrate with membrane for 1 minute, remove excess solution (membrane remains wet), wrap in plastic, and expose to X-ray film.
    ​​​​​​​*Avoid repeated exposure to skin (see enclosed Material Safety Data Sheet or refer to our website for further information).

    Solutions and Reagents

    Each SignalFire™ ECL Reagent (A and B) is a 2X concentrate; there is no need to further dilute in water when the two reagents are combined.

    Storage

    SignalFire™ ECL Reagent is stable at room temperature. DO NOT store Reagents A and B pre-mixed. Reagents A and B should be combined just prior to exposing membranes.

    Product Description

    SignalFire™ ECL Reagent from Cell Signaling Technology (CST) is an enhanced chemiluminescent substrate capable of detecting picogram amounts of protein by western blot analysis. Compared to entry-substrates, SignalFire™ ECL Reagent boasts a more robust signal and extended duration of signal output. In the presence of hydrogen peroxide, horseradish peroxidase (HRP) converts luminol to an excited intermediate dianion. This dianion emits light on return to its ground state. Light emission is maximal immediately after exposure of the substrate to HRP and continues for 1 hour. Light can be captured on X-ray film, typically by exposure for a few seconds. Maximum sensitivity can be obtained with longer exposure.

    Background

    Chemiluminescence systems have emerged as the best all-around method for western blot detection. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on film, it is possible to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times.

      Limited Uses

      Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

      Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

      For Research Use Only. Not For Use In Diagnostic Procedures.
      Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
      SignalFire is a registered trademark of Cell Signaling Technology, Inc.
      All other trademarks are the property of their respective owners. Visit our Trademark Information page.