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XML generation date: 2024-12-18 19:01:07.241
Product last modified at: 2025-01-01T09:02:29.521Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Normal Mouse IgG #68860

Filter:
  • C&R
  • C&T
CUT & RUN Image 1: Normal Mouse IgG
CUT&RUN was performed with HeLa cells and Rpb1 CTD (4H8) Mouse mAb #2629 or Normal Mouse IgG, using CUT&RUN Assay Kit #86652. DNA Libraries were prepared using DNA Library Prep Kit for Illumina® (ChIP-seq, CUT&RUN) #56795. The figures show binding across GAPDH, a known target gene of Rpb1 (see additional figure containing CUT&RUN-qPCR data).

To Purchase # 68860

Cat. # Size Qty. Price Ships
68860L 100 µl
$146 Jan 22

Supporting Data

REACTIVITY
SENSITIVITY
MW (kDa)
SOURCE Mouse
Application Key:
  • C&R-CUT & RUN 
  • C&T-CUT & Tag 

Product Information

Product Description

Normal Mouse IgG is an unconjugated mouse polyclonal antibody that is routinely used as a non-specific IgG control and works optimally with the Cell Signaling Technology® CUT&Tag protocol.

Product Usage Information

The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652. The CUT&Tag dilution was determined using CUT&Tag Assay Kit #77552.

Application Dilution
CUT&RUN 1:50
CUT&Tag 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody. This product is stable for 24 months when stored at –20°C.

Protocol

Source / Purification

The purified antibody is not directed against any known antigen. It was isolated from pooled mouse sera.

Background

Normal Mouse IgG is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
    For Research Use Only. Not For Use In Diagnostic Procedures.
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