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Product last modified at: 2025-01-01T09:02:09.758Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Sox10 (E6B6I) XP® Rabbit mAb #69661

Filter:
  • WB
  • IP
  • IHC
Western Blotting Image 1: Sox10 (E6B6I) XP® Rabbit mAb
Western blot analysis of extracts from SK-MEL-28 and HeLa cells using Sox10 (E6B6I) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, HeLa is low or negative for Sox10 expression.

To Purchase # 69661

Cat. # Size Qty. Price Ships
69661T 20 µl
$172
69661S 100 µl
$402

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 62
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
  • IHC-Immunohistochemistry 
Species Cross-Reactivity Key:
  • H-Human 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:100
IHC Leica Bond 1:50 - 1:200
Immunohistochemistry (Paraffin) 1:50 - 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #88386.

Protocol

Specificity / Sensitivity

Sox10 (E6B6I) XP® Rabbit mAb recognizes endogenous levels of total Sox10 protein. This antibody also recognizes presumptive sumoylated forms of Sox10 protein. Non-specific staining was observed in neuroendocrine cells of the stomach by immunohistochemistry.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly446 of human Sox10 protein.

Background

SRY-box 10 (Sox10) is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and the determination of cell fate. Sox10 is an important regulator of neural crest and peripheral nervous system development (1-3). Mutations in the SOX10 gene are associated with a group of auditory-pigmentary developmental disorders, including Waardenburg and Waardenburg-Shah syndromes (3,4). Research studies suggest an oncogenic role for Sox10 in various tumor types, such as hepatocellular carcinoma and melanoma (5,6).
For Research Use Only. Not For Use In Diagnostic Procedures.
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