20X LumiGLO® Reagent and 20X Peroxide #7003
To Purchase # 7003
| Cat. # | Size | Qty. | Price |
|---|---|---|---|
| 7003P | 10 ml 5 ml each substrate | $87 | |
| 7003S | 50 ml 25 ml each substrate | $259 |
- Product Includes
- Related Products
Kit Includes | Petite Kit (P) Quantity | Small Kit (S) Quantity |
|---|---|---|
LumiGLO® Reagent A (20X) | 1 x 5 ml | 1 x 25 ml |
Peroxide Reagent B (20X) | 1 x 5 ml | 1 x 25 ml |
Product Information
Product Usage Information
Prepare solutions with Milli-Q® or equivalently purified water.
1. Wash membrane-bound HRP (antibody conjugate) three times, for 5 minutes in TBS/T (20 mM Tris-HCl (pH 7.6),137 mM NaCl and 0.1% Tween-20)
2. Prepare substrate by diluting 20X LumiGLO® and 20X Peroxide to 1X in water (e.g. for 10 ml, add 0.5 ml LumiGLO® and 0.5 ml peroxide to 9.0 ml water).
3. Incubate substrate with membrane for 1 minute, remove excess solution (membrane remains wet), wrap in plastic and expose to X-ray film.
Storage
Product Description
Advantages of the Phototope® Western Detection System:
Sensitivity: Detection of sub-picogram amounts of protein is routine with good primary antisera.
Speed: Less than 1 hour is required for the entire detection procedure. Exposure times are seconds to minutes.
Multiple Exposures: Light is emitted at a constant rate for several minutes, so you can perform multiple exposures to optimize signal intensity. Re-exposure at a future date is achieved by simply adding more reagent.
*Avoid repeated exposure to skin (see enclosed Material Safety Data Sheet or refer to our website for further information).
Background
Alternate Names
20X LumiGLO® Reagent and 20X Peroxide; chemiluminescence; ECL; Luminol
Limited Uses
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