FXR/NR1H4 (E4B8P) Mouse mAb #72105
- WB
- IP
- ChIP
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 55 |
| Source/Isotype | Mouse IgG2a |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
| Chromatin IP | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
- Parks, D.J. et al. (1999) Science 284, 1365-8.
- Wang, H. et al. (1999) Mol Cell 3, 543-53.
- Laffitte, B.A. et al. (2000) J Biol Chem 275, 10638-47.
- Edwards, P.A. et al. (2002) J Lipid Res 43, 2-12.
- Goodwin, B. et al. (2000) Mol Cell 6, 517-26.
- Ma, K. et al. (2006) J Clin Invest 116, 1102-9.
- Zhang, Y. et al. (2006) Proc Natl Acad Sci U S A 103, 1006-11.
- Tarling, E.J. et al. (2017) J Clin Invest 127, 3741-54.
- Gomez-Ospina, N. et al. (2016) Nat Commun 7, 10713.
- Modica, S. et al. (2008) Cancer Res 68, 9589-94.
- Fu, T. et al. (2019) Cell 176, 1098-1112.e18.
- Qiao, P. et al. (2018) Oncol Rep 40, 2067-78.
- Pellicciari, R. et al. (2002) J Med Chem 45, 3569-72.
- Hirschfield, G.M. et al. (2015) Gastroenterology 148, 751-61.e8.
- Neuschwander-Tetri, B.A. et al. (2015) Lancet 385, 956-65.
- Genin, M.J. et al. (2015) J Med Chem 58, 9768-72.
Limited Uses
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