SignalStain® Deep Black Peroxidase Substrate Kit #72986
Product Information
Product Usage Information
2. After incubation with SignalStain® Boost IHC Detection Reagent or other HRP-based detection systems, wash slides and incubate with the working solution at room temperature until staining develops. 5-10 minutes generally provides an acceptable staining intensity.
3. Wash slides for 5 minutes in water.
4. Counterstain, if desired. Deep Black/HRP chromogen-substrate is compatible with Hematoxylin #14166.
5. Slides can be dehydrated through alcohol and cleared with xylene or xylene substitute or can be air dried, then permanently mounted with SignalStain® Aqueous Mounting Medium #27290 or SignalStain® Mounting Medium #14177.
For best color preservation and long-term slide storage, it is recommended to use SignalStain® Mounting Prep Solution #50815.
Appropriate personal protective equipment should be worn when handling Deep Black. Dispose of in accordance with local regulations.
Storage
Product Description
Limited Uses
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