DDX21 Antibody #75762
- WB
- IP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 100 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala780 of human DDX21 protein. Antibodies are purified by peptide affinity chromatography.
Background
DDX21, also known as RNA helicase II/Gu, is an RNA helicase that plays important roles in transcription and ribosomal RNA (rRNA) processing (1-3). DDX21 associates with many RNA Polymerase I and II genes, including the direct binding to the rDNA locus to promote ribosome biogenesis. The helicase activity of DDX21 contributes to transcription by helping release P-TEFb from the 7SK small nuclear ribonucleoprotein (snRNP) complex (3). Nucleolar localization of DDX21 has been shown to be regulated through phosphorylation by JNK and interaction with c-Jun. Depletion of either c-Jun or JNK has shown to impair rRNA processing (4,5). DDX21 can also become acetylated by CBP, which reduces its activity. Deacetylation by SIRT7 increases DDX21 helicase activity to prevent R-loop accumulation and protect genomic stability (6). DDX21 can also form a complex with DDX1, DHX36, and TRIF to sense dsRNA, which in turn activates interferon response in innate immunity (7,8).
- Yang, H. et al. (2003) J Biol Chem 278, 38847-59.
- Henning, D. et al. (2003) J Biol Chem 278, 52307-14.
- Calo, E. et al. (2015) Nature 518, 249-53.
- Holmström, T.H. et al. (2008) J Biol Chem 283, 7046-53.
- Mialon, A. et al. (2008) FEBS Lett 582, 3145-51.
- Song, C. et al. (2017) Genes Dev 31, 1370-1381.
- Zhang, Z. et al. (2011) Immunity 34, 866-78.
- Tsai, S.Y. et al. (2014) PLoS Pathog 10, e1003848.
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