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Render Timestamp: 2024-10-25T09:56:13.261Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-09-30 01:56:27.934
Product last modified at: 2024-09-30T08:00:37.965Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. FAT10 (D1Q3Y) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

FAT10 (D1Q3Y) Rabbit mAb #76194

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 18
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    FAT10 (D1Q3Y) Rabbit mAb recognizes endogenous levels of total FAT10 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human FAT10 protein.

    Background

    HLA-F adjacent transcript 10 (FAT10/Ubiquitin D) belongs to the ubiquitin-like modifier (Ubl) family of proteins. The 18 kDa FAT10 protein contains two tandem Ubl domains that are oriented in a head-to-tail fashion and a free C-terminal di-glycine motif, which is available for isopeptide bond formation with target proteins via an E1-E2-E3 enzymatic cascade (1). Indeed, FAT10 provides a ubiquitin-independent signal for proteasomal degradation (2). Research studies have demonstrated that FAT10 expression is enriched in lymphoid organs and that its expression is transiently upregulated via the NF-kB pathway in response to pro-inflammatory cytokines such as TNFα and IFNγ (1,3-5). In solid tumors that possess inflammatory microenviroments, research studies have shown that FAT10 is overexpressed and may serve as a biomarker for inflamed tumors (3,4).

    Database Links

    UniProt ID: O15205


    Entrez-Gene Id: 10537


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    For Research Use Only. Not For Use In Diagnostic Procedures.
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