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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

MDM2 (D1V2Z) Rabbit mAb (Biotinylated) #76794

Filter:
  • WB
Western Blotting Image 1: MDM2 (D1V2Z) Rabbit mAb (Biotinylated)
Western blot analysis of extracts from U-2 OS cells, untreated (-) or treated with Nutlin 3a (10 μM, 24 hr; +), using MDM2 (D1V2Z) Rabbit mAb (Biotinylated) (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

To Purchase # 76794

Cat. # Size Qty. Price
76794S 100 µl
$450

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 90
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Description

This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated MDM2 (D1V2Z) Rabbit mAb #86934.
MW (kDa) 90

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 136 mM NaCl, 2.6 mM KCI, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

MDM2 (D1V2Z) Rabbit mAb (Biotinylated) recognizes endogenous levels of total MDM2 protein. Based on the sequence of the peptide antigen, this antibody is expected to detect full-length MDM2, as well as isoforms A, C, alpha, F, G, and 11. This antibody may detect isoform A1.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val280 of human MDM2 protein.

Background

MDM2, a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 (1). Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53 (2-4). Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53 (3).

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
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