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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

MCRIP1 (D2Y8V) Rabbit mAb #78128

Filter:
  • WB
  • IP
Western Blotting Image 1: MCRIP1 (D2Y8V) Rabbit mAb
Western blot analysis of extracts from various cell lines using MCRIP1 (D2Y8V) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

To Purchase # 78128

Cat. # Size Qty. Price Ships
78128T 20 µl
$153 Jan 23
78128S 100 µl
$339 Jan 23

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 11
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

MCRIP1 (D2Y8V) Rabbit mAb recognizes endogenous levels of total MCRIP1 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro56 of human MCRIP1 protein.

Background

Mapk-regulated corepressor-interacting protein 1 (MCRIP1) is a widely expressed protein substrate of ERK. MCRIP1 functions in regulating transcriptional repression of E-cadherin by the transcriptional corepressor CtBP. Unphosphorylated MCRIP1 binds to CtBP, preventing its transcriptional regulatory activity. When MCRIP1 is phosphorylated by ERK, CtIP is free to interact with the transcriptional repressor, ZEB1. The CtIP/ZEB1 complex then silences E-cadherin expression, promoting epithelial-mesenchymal transition (EMT), a process in tumor progression that leads to enhanced migration and invasion (1,2).
For Research Use Only. Not For Use In Diagnostic Procedures.
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