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XML generation date: 2025-03-07 13:18:22.687
Product last modified at: 2025-03-08T08:19:27.678Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

OCA-T1 (E4T1K) Rabbit mAb (BSA and Azide Free) #78954

Filter:
  • WB
  • IHC
Western Blotting Image 1: OCA-T1 (E4T1K) Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from COR-L311, NCI-H526, and HeLa cells using OCA-T1 (E4T1K) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Data were generated using the standard formulation of this product.

To Purchase # 78954

Cat. # Size Qty. Price
78954SF 100 µg
$843

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 30
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IHC-Immunohistochemistry 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Usage Information

This product is the carrier free version of product #49316. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

Formulation

Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

For standard formulation of this product see product #49316

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

OCA-T1 (E4T1K) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total OCA-T1 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro150 of human OCA-T1 protein.

Background

OCA-T1 is encoded by the gene POU2AF2 (C11orf53), which was identified in a single-cell RNA sequencing study to be expressed selectively in tuft cells from mouse and human tissues (1). The expression of OCA-T1 was observed to occur in a pattern strikingly similar to that of POU2F3, a master regulator of tuft cell development whose expression also defines a tuft cell-like variant (SCLC-P) of small cell lung cancers (2). Although containing no recognizable structural domains, OCA-T1 showed minor sequence similarity with OCA-B, a protein encoded by the POU2AF1 gene and reported to be an activator of selected Class II POU domain transcription factors (3). Functional studies revealed that OCA-T1 forms a protein complex with POU2F3; these proteins furthermore share genomic binding sites and promote the expression of genes directly associated with tuft cell development. Lastly, Crispr/Cas9-mediated deletion of POU2AF2 in mice revealed that OCA-T1 is functionally required for normal tuft cell development (1). Collectively, these findings suggest that OCA-T1 is a critical component of the POU2F3-containing transcriptional complex that regulates the expression of genes governing tuft cell development.
For Research Use Only. Not For Use In Diagnostic Procedures.
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