HJURP (D3A8Z) Rabbit mAb #80508
- WB
Supporting Data
REACTIVITY | H |
SENSITIVITY | Endogenous |
MW (kDa) | 90 |
Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly672 of human HJURP protein.
Background
CENP-A is an essential histone H3 variant that replaces canonical histone H3 in centromeric heterochromatin. The inherited localization of the centromere is specified by CENP-A (1). CENP-A deposition to the correct chromosomal location in early G1 phase is regulated by the Mis18 complex, which recruits the CENP-A assembly factor/chaperone protein HJURP (Holliday Junction Recognition Protein) (2-3).
Dimerization of HJURP is required for its activity (4), and phosphorylation by cyclin dependent kinases is required for temporal regulation of HJURP recruitment (5).
Overexpression of HJURP causes chromosome loss in yeast and mitotic defects in mammalian cells (6). Further, downregulation of HJURP expression has been associated with replicative senescence in human cells (7).
Research studies indicate that HJURP may have prognostic value in human breast cancer and high grade gliomas (8-10).
- Ausió, J. (2006) Brief Funct Genomic Proteomic 5, 228-43.
- Stellfox, M.E. et al. (2013) Cell Mol Life Sci 70, 387-406.
- Wang, J. et al. (2014) J Biol Chem 289, 8326-36.
- Zasadzińska, E. et al. (2013) EMBO J 32, 2113-24.
- Müller, S. et al. (2014) Cell Rep 8, 190-203.
- Mishra, P.K. et al. (2011) PLoS Genet 7, e1002303.
- Heo, J.I. et al. (2013) J Gerontol A Biol Sci Med Sci 68, 914-25.
- Hu, Z. et al. (2010) Breast Cancer Res 12, R18.
- Montes de Oca, R. et al. (2015) Mol Oncol 9, 657-74.
- de Tayrac, M. et al. (2013) PLoS One 8, e73332.
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.