Render Target: STATIC
Render Timestamp: 2025-01-21T11:05:34.605Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-11-18 16:03:13.238
Product last modified at: 2025-01-17T12:30:16.486Z
Cell Signaling Technology Logo

Basket Updated

0

Items added

1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

MCT4/SLC16A3 (F2K6A) Rabbit mAb #81569

Filter:
  • WB
Western Blotting Image 1: MCT4/SLC16A3 (F2K6A) Rabbit mAb
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged human MCT4/SLC16A3 (hMCT4/SLC16A3-Myc/DDK; +), using MCT4/SLC16A3 (F2K6A) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).

To Purchase # 81569

Cat. # Size Qty. Price Ships
81569T 20 µl
$155
81569S 100 µl
$371

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 40
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

MCT4/SLC16A3 (F2K6A) Rabbit mAb recognizes endogenous levels of total MCT4/SLC16A3 protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human MCT4/SLC16A3 protein.

Background

MCT4, also known as MOT4 or SLC16A3, is a multi-pass transmembrane monocarboxylate transporter (MCT) protein encoded by the gene SLC16A3 (1,2). MCT4 is reported to be expressed in white (glycolytic) muscle cells (3,4), astrocytes (5), activated macrophages (6), and cancer cells (2). The primary role of MCT4 is lactate exportation, which is adapted to the release of lactate from glycolytic cells (8,9). In the tumor microenvironment, MCT4 mediated lactate secretion promotes M2 macrophage polarization and suppresses T cell function (10). MCT4 inhibition increases the efficacy of immune checkpoint blockade and is a potential target to reverse tumor resistance to immunotherapy (11,12).
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.