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Product last modified at: 2025-01-03T15:00:10.378Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Sox17 (D1T8M) Rabbit mAb #81778

Filter:
  • WB
  • IF
Western Blotting Image 1: Sox17 (D1T8M) Rabbit mAb
Western blot analysis of extracts from various cell lines using Sox17 (D1T8M) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Expression in HeLa cells is very low, as expected.

To Purchase # 81778

Cat. # Size Qty. Price Ships
81778T 20 µl
$153
81778S 100 µl
$339

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 55
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IF-Immunofluorescence 
Species Cross-Reactivity Key:
  • H-Human 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunofluorescence (Immunocytochemistry) 1:3200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Sox17 (D1T8M) Rabbit mAb recognizes endogenous levels of total Sox17 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro272 of human Sox17 protein.

Background

SRY-related high mobility group box (SOX) proteins comprise a large family of widely conserved transcription factors that play important roles in development. SOX proteins possess a high mobility group (HMG) motif that binds the DNA minor groove. SOX proteins do not directly mediate transcription, but require binding partners that regulate their ability to mediate transcription of target genes that control cell fate determination and development (reviewed in 1).

Transcription factor Sox17 is a Sox family protein with an established role in endoderm specification during development (2). In addition, Sox17 plays essential roles in the maintenance of the hematopoietic stem cell pool (3) and for vascular morphogenesis during development (4,5). Sox17 negatively regulates oligodendrocyte precursor differentiation by antagonizing β-catenin signaling (6). Mutation in the corresponding Sox17 gene in humans is associated with a form of vesicoureteral reflux, a disorder characterized by congenital kidney and urinary tract defects (7).
For Research Use Only. Not For Use In Diagnostic Procedures.
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