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Render Timestamp: 2024-11-21T14:01:01.504Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:57:21.857
Product last modified at: 2024-09-30T08:00:51.846Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. PBRM1/BAF180 (E6N2K) Mouse mAb

PBRM1/BAF180 (E6N2K) Mouse mAb #81832

Filter:
  • WB
  • ChIP

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 205
    Source/Isotype Mouse IgG2a kappa
    Application Key:
    • WB-Western Blotting 
    • ChIP-Chromatin Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 × 10^6 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.

    Application Dilution
    Western Blotting 1:1000
    Chromatin IP 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    PBRM1/BAF180 (E6N2K) Mouse mAb recognizes endogenous levels of total PBRM1/BAF180 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human PBRM1/BAF180 protein.

    Background

    ATP-dependent chromatin remodeling complexes play an essential role in the regulation of various nuclear processes, such as gene expression, DNA replication, and repair (1,2). The SWI/SNF chromatin remodeling complex consists of more than 10 subunits with a single molecule of the ATPase catalytic subunit BRM or BRG1, but not both. The activities of these two subunits drive the disruption of histone-DNA contacts that lead to changes in accessibility of crucial regulatory elements within chromatin (2-5). The BRM/BRG1 containing SWI/SNF complexes are recruited to target promoters by transcription factors, such as nuclear receptors, p53, RB, and BRCA1 to regulate gene activation, cell growth, the cell cycle, and differentiation processes (1,6-9).

    PBRM1/BAF180 is a unique member of the SWI/SNF complex PBAF, which binds to kinetochores in mitotic chromatin (10,11). PBAF is involved in nuclear receptor-mediated transcription and retinoic acid driven gene activation (12,13). PBRM1/BAF180 has been shown to be a potent tumor suppressor, as it is the second-most mutated gene in renal carcinomas (14). Mutations of PBRM1/BAF180 have also been shown to be involved in breast cancer, and low expression relates to poorer prognosis (15,16). PBRM1/BAF180 is phosphorylated at Ser948 by ATM during DNA damage, which is important for transcriptional silencing and repair around double-stranded breaks (17).

    test
    1. Ho, L. and Crabtree, G.R. (2010) Nature 463, 474-84.
    2. Becker, P.B. and Hörz, W. (2002) Annu Rev Biochem 71, 247-73.
    3. Eberharter, A. and Becker, P.B. (2004) J Cell Sci 117, 3707-11.
    4. Bowman, G.D. (2010) Curr Opin Struct Biol 20, 73-81.
    5. Gangaraju, V.K. and Bartholomew, B. (2007) Mutat Res 618, 3-17.
    6. Lessard, J.A. and Crabtree, G.R. (2010) Annu Rev Cell Dev Biol 26, 503-32.
    7. Morettini, S. et al. (2008) Front Biosci 13, 5522-32.
    8. Wolf, I.M. et al. (2008) J Cell Biochem 104, 1580-6.
    9. Simone, C. (2006) J Cell Physiol 207, 309-14.
    10. Nie, Z. et al. (2000) Mol Cell Biol 20, 8879-88.
    11. Xue, Y. et al. (2000) Proc Natl Acad Sci U S A 97, 13015-20.
    12. Lemon, B. et al. (2001) Nature 414, 924-8.
    13. Wang, Z. et al. (2004) Genes Dev 18, 3106-16.
    14. Varela, I. et al. (2011) Nature 469, 539-42.
    15. Xia, W. et al. (2008) Cancer Res 68, 1667-74.
    16. Mo, D. et al. (2015) Int J Clin Exp Pathol 8, 9307-13.
    17. Kakarougkas, A. et al. (2014) Mol Cell 55, 723-32.

    Database Links

    UniProt ID: Q86U86


    Entrez-Gene Id: 55193


    Limited Uses

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    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
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