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R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Phospho-Chk2 (Thr68) (E8Q1A) Rabbit mAb #82263

Filter:
  • WB
  • IHC
  • F
Western Blotting Image 1: Phospho-Chk2 (Thr68) (E8Q1A) Rabbit mAb
Western blot analysis of extracts from HCT 116 cells, untreated (-) or treated with neocarzinostatin (0.5 µg/mL, 1 hr; +), using Phospho-Chk2 (Thr68) (E8Q1A) Rabbit mAb (upper), Chk2 (D9C6) XP® Rabbit mAb #6334 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower).

To Purchase # 82263

Cat. # Size Qty. Price
82263T 20 µl
$186
82263S 100 µl
$414

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 62
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IHC-Immunohistochemistry 
  • F-Flow Cytometry 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products
  • Conjugates

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunohistochemistry (Paraffin) 1:75 - 1:300
Flow Cytometry (Fixed/Permeabilized) 1:200 - 1:800

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #64180.

Protocol

Specificity / Sensitivity

Phospho-Chk2 (Thr68) (E8Q1A) Rabbit mAb recognizes endogenous levels of Chk2 protein only when phosphorylated at Thr68. Non-specific non-nuclear staining was observed in smooth and skeletal muscle by immunohistochemistry.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr68 of human Chk2 protein.

Background

Chk2 is the mammalian orthologue of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases (1-3). The amino-terminal domain of Chk2 contains a series of seven serine or threonine residues (Ser19, Thr26, Ser28, Ser33, Ser35, Ser50, and Thr68) each followed by glutamine (SQ or TQ motif). These are known to be preferred sites for phosphorylation by ATM/ATR kinases (4,5). After DNA damage by ionizing radiation (IR), UV irradiation, or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR (5-7). The SQ/TQ cluster domain, therefore, seems to have a regulatory function. Phosphorylation at Thr68 is a prerequisite for the subsequent activation step, which is attributable to autophosphorylation of Chk2 at residues Thr383 and Thr387 in the activation loop of the kinase domain (8).

Pathways

Explore pathways related to this product.


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