Render Target: STATIC
Render Timestamp: 2025-01-07T12:12:57.528Z
Commit: 199712eb9daea12d88cc0e67894a8a09f475f8cb
XML generation date: 2024-09-30 01:58:36.127
Product last modified at: 2025-01-01T09:03:46.820Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

ZNF217 (E6X4R) Rabbit mAb #82306

Filter:
  • WB
  • IP
  • IF
  • ChIP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 140
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    • ChIP-Chromatin Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Immunofluorescence (Immunocytochemistry) 1:800 - 1:1600
    Chromatin IP 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ZNF217 (E6X4R) Rabbit mAb recognizes endogenous levels of total ZNF217 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ZNF217 protein.

    Background

    Zinc finger protein 217 (ZNF217) is an oncogenic transcription factor that regulates the expression of a wide variety of genes. ZNF217 is a member of the Kruppel-like factor (KLF) family of transcription factors, which consists of zinc finger proteins that bind to CACCC or GT box DNA elements (1). ZNF217 regulates the expression of its target genes through direct DNA binding, and through interaction with transcriptional co-repressors in the CoREST-HDAC complex (2). Although it was initially described as a transcriptional repressor, ZNF217 may also act as a transcriptional activator depending on the target gene (3). Amplification of the ZNF217 gene itself has been observed in a variety of tumors, where it is typically associated with poor prognosis (4-7). Overexpression of ZNF217 protein leads to increased cell proliferation and promotes tumor growth in breast and ovarian cancer (8,9). ZNF217 also interferes with apoptotic signaling and confers resistance to chemotherapeutic drugs in breast cancer (9,10).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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