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Product last modified at: 2025-01-03T21:30:09.199Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

ARID2 (D8D8U) Rabbit mAb #82342

Filter:
  • WB
  • IP
Western Blotting Image 1: ARID2 (D8D8U) Rabbit mAb
Western blot analysis of extracts from various cell lines using ARID2 (D8D8U) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).

To Purchase # 82342

Cat. # Size Qty. Price Ships
82342T 20 µl
$153
82342S 100 µl
$339

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 220
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

ARID2 (D8D8U) Rabbit mAb recognizes endogenous levels of total ARID2 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val891 of human ARID2 protein.

Background

ATP-dependent chromatin remodeling complexes play an essential role in the regulation of various nuclear processes, such as gene expression, DNA replication, and repair (1,2). The SWI/SNF chromatin remodeling complex consists of more than 10 subunits with a single molecule of the ATPase catalytic subunit BRM or BRG1, but not both. The activities of these two subunits drive the disruption of histone-DNA contacts that lead to changes in accessibility of crucial regulatory elements within chromatin (2-5). The BRM/BRG1 containing SWI/SNF complexes are recruited to target promoters by transcription factors, such as nuclear receptors, p53, RB, and BRCA1 to regulate gene activation, cell growth, the cell cycle, and differentiation processes (1,6-9).

ARID2 is a unique member of the SWI/SNF complex PBAF, which binds to kinetochores in mitotic chromatin (10,11). PBAF is involved in nuclear receptor-mediated transcription and retinoic acid driven gene activation (12,13). ARID2 is the targeting subunit of the PBAF complex and critical for complex stability (14). It can also mediate DNA repair of double stranded breaks through interactions with RAD51 (15). ARID2 also has been demonstrated to be a tumor suppressor, and inactivating mutations have been found in various cancer types (16-18).
  1. Ho, L. and Crabtree, G.R. (2010) Nature 463, 474-84.
  2. Becker, P.B. and Hörz, W. (2002) Annu Rev Biochem 71, 247-73.
  3. Eberharter, A. and Becker, P.B. (2004) J Cell Sci 117, 3707-11.
  4. Bowman, G.D. (2010) Curr Opin Struct Biol 20, 73-81.
  5. Gangaraju, V.K. and Bartholomew, B. (2007) Mutat Res 618, 3-17.
  6. Lessard, J.A. and Crabtree, G.R. (2010) Annu Rev Cell Dev Biol 26, 503-32.
  7. Morettini, S. et al. (2008) Front Biosci 13, 5522-32.
  8. Wolf, I.M. et al. (2008) J Cell Biochem 104, 1580-6.
  9. Simone, C. (2006) J Cell Physiol 207, 309-14.
  10. Nie, Z. et al. (2000) Mol Cell Biol 20, 8879-88.
  11. Xue, Y. et al. (2000) Proc Natl Acad Sci U S A 97, 13015-20.
  12. Lemon, B. et al. Nature 414, 924-8.
  13. Wang, Z. et al. (2004) Genes Dev 18, 3106-16.
  14. Yan, Z. et al. (2005) Genes Dev 19, 1662-7.
  15. de Castro, R.O. et al. (2017) J Biol Chem 292, 8459-8471.
  16. Li, M. et al. (2011) Nat Genet 43, 828-9.
  17. Hillen, U. et al. (2016) Am J Surg Pathol 40, 1721-1723.
  18. Manceau, G. et al. (2013) Int J Cancer 132, 2217-21.
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