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Render Timestamp: 2025-01-10T12:01:18.508Z
Commit: 199712eb9daea12d88cc0e67894a8a09f475f8cb
XML generation date: 2024-09-30 01:59:37.706
Product last modified at: 2025-01-01T09:01:43.272Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. ACSL3 (E2S9L) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

ACSL3 (E2S9L) Rabbit mAb #83319

Filter:
  • WB
  • IP
  • IF

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 79
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100
    Immunofluorescence (Immunocytochemistry) 1:200 - 1:800

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ACSL3 (E2S9L) Rabbit mAb recognizes endogenous levels of total ACSL3 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu529 of human ACSL3 protein.

    Background

    Mammalian long-chain acyl-CoA synthetase (LACS or ACSL) catalyzes the ligation of fatty acids to CoA to form fatty acyl-CoA in a two-step reaction. Five isoforms of ACSL have been identified. These isoforms have different substrate preferences and subcellular localizations (1). Studies show that exogenous monounsaturated fatty acids inhibit ferroptosis and that this process requires the activation of monounsaturated fatty acids by acyl-CoA synthetase long-chain family member 3 (ACSL3) (2). In addition, studies on a mouse model show that Acsl3 is essential for oleic acid to protect melanoma cells from ferroptosis, which leads to more tumor metastases (3). Furthermore, ACSL3 is required for prostaglandin synthesis and tumorigenesis in non-small cell lung cancer (NSCLC) (4).

    Database Links

    UniProt ID: O95573


    Entrez-Gene Id: 2181


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    For Research Use Only. Not For Use In Diagnostic Procedures.
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