CD62P/P-Selectin (E8Q1W) XP® Rabbit mAb #84298
- WB
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 140 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| IHC Leica Bond | 1:400 - 1:1600 |
| Immunohistochemistry (Paraffin) | 1:200 - 1:800 |
Storage
For a carrier free (BSA and azide free) version of this product see product #31214.
Protocol
Specificity / Sensitivity
CD62P/P-Selectin (E8Q1W) XP® Rabbit mAb recognizes endogenous levels of total CD62P/P-Selectin protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu433 of human CD62P/P-Selectin protein.
Background
CD62P/P-Selectin is a type-I transmembrane glycoprotein that is largely expressed and stored in intracellular granules by resting platelets, megakaryocytes, and vascular endothelial cells (1,2). The restricted expression pattern of CD62P/P-Selectin positions this molecule as a key regulator of hemostasis, thrombosis, and inflammation. Platelets and endothelial cells that become activated in response to proinflammatory cytokines express CD62P/P-Selectin on the cell surface, which serves as an adhesion receptor to facilitate recruitment of leukocytes to sites of inflammation (3-5). CD62P/P-Selectin has also been implicated in cancer cell metastasis by promoting the adhesion of tumor cells in circulation to activated platelets and endothelial cells (6,7).
- Stenberg, P.E. et al. (1985) J Cell Biol 101, 880-6.
- Johnston, G.I. et al. (1989) J Biol Chem 264, 1816-23.
- Pouyani, T. and Seed, B. (1995) Cell 83, 333-43.
- Somers, W.S. et al. (2000) Cell 103, 467-79.
- Hattori, R. et al. (1989) J Biol Chem 264, 7768-71.
- Erpenbeck, L. and Schön, M.P. (2010) Blood 115, 3427-36.
- Köhler, S. et al. (2010) Br J Cancer 102, 602-9.
Limited Uses
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