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Render Timestamp: 2024-11-21T13:48:06.470Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-20 06:18:51.458
Product last modified at: 2024-10-16T20:45:13.234Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

CD86/B7-2 (GL-1) Rat mAb (APC Conjugate) #84393

Filter:
  • F

    Supporting Data

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rat IgG2a kappa
    Application Key:
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Description

    This Cell Signaling Technology antibody is conjugated to APC and tested in-house for direct flow cytometric analysis in mouse cells.

    Product Usage Information

    For optimal flow cytometry results, we recommend 0.06 μg of antibody per test.

    Application Dilution
    Flow Cytometry (Fixed/Permeabilized) 1:330
    Flow Cytometry (Live) 1:330

    Storage

    Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.

    Protocol

    Specificity / Sensitivity

    CD86/B7-2 (GL-1) Rat mAb (APC Conjugate) recognizes endogenous levels of total CD86/B7-2 protein. This antibody detects an epitope within the extracellular domain.

    Species Reactivity:

    Mouse

    Source / Purification

    This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

    Background

    CD80 (B7-1, BB1) and CD86 (B7-2, B70) are members of the B7 family of cell surface ligands that regulate T cell activation and immune responses. CD80 is expressed on activated antigen presenting cells, including dendritic cells, B cells, monocytes, and macrophages. CD86 is expressed on resting monocytes, dendritic cells, activated B lymphocytes, and can be further upregulated in the presence of inflammation (1-3). CD80 and CD86 are ligands for CD28, which functions as a T cell costimulatory receptor. Interaction of CD28 with CD80 or CD86 provides the second signal required for naïve T cell activation, T cell proliferation, and acquisition of effector functions (3-7). Alternatively, CD80 and CD86 also act as ligands to CTLA-4, which results in the downregulation of T cell activity (3,7-9).
    The GL-1 antibody is used as a marker for CD86 expression on B cells, macrophages, and dendritic cells.
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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