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Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-09-25 17:06:08.462
Product last modified at: 2025-01-08T15:45:08.033Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

CCL3/MIP-1α (F2M8Q) Rabbit mAb #85270

Filter:
  • WB
  • IHC
Western Blotting Image 1: CCL3/MIP-1α (F2M8Q) Rabbit mAb
Western blot analysis of extracts from RAW 264.7 and C2C12 cells, untreated (-) or treated with Lipopolysaccharide (LPS) #14011 (100 ng/mL, 7 hr; +), using CCL3/MIP-1α (F2M8Q) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). CCL3/MIP-1α is induced by LPS treatment as expected.

To Purchase # 85270

Cat. # Size Qty. Price Ships
85270T 20 µl
$165
85270S 100 µl
$402

Supporting Data

REACTIVITY M
SENSITIVITY Endogenous
MW (kDa) 10
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IHC-Immunohistochemistry 
Species Cross-Reactivity Key:
  • M-Mouse 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunohistochemistry (Paraffin) 1:200 - 1:800

Protocol

Specificity / Sensitivity

CCL3/MIP-1α (F2M8Q) Rabbit mAb recognizes endogenous levels of total CCL3/MIP-1α protein. This antibody detects a 42 kDa band of unknown origin in some cell lines.

Species Reactivity:

Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse CCL3/MIP-1α protein.

Background

Chemokine (C-C motif) ligand 3 (CCL3), also known as macrophage inflammatory protein-1α (MIP-1α), is a 10 kDa member of the C-C or β family of chemokines that play key roles in immune surveillance, inflammation, and infection (1). CCL3 shares 68% amino acid identity with CCL4 (MIP-1β) and 94% amino acid identity with LD78β (1). CCL3 is produced by activated monocytes, activated macrophages, NK cells, and T cells (1). There are two receptors for CCL3: CCR1 and CCR5 (1). CCL3 inhibits R5 HIV-1 infection by competing with the virus for its co-receptor, CCR5 (1). CCL3 possesses hematopoietic stem cell inhibitory activity, suppressing their proliferation, but has been shown to promote proliferation of more mature progenitors (2-4). CCL3 has been implicated in several forms of leukemia, in which malignant cells may lose responsiveness to CCL3 or produce high levels of CCL3 (2). CCL3 polymorphisms are associated with risk for the development of rheumatoid arthritis and ulcerative colitis (5,6). CCL3 expression is upregulated in the brains of patients with Alzheimer's disease (AD) with dementia, and reduced expression of CCL3 is observed in AD patients resilient to dementia (7).
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