COX IV (3E11) Rabbit mAb (Alexa Fluor® 594 Conjugate) #8692
- IF
Supporting Data
| REACTIVITY | H R Mk Z B Pg |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- R-Rat
- Mk-Monkey
- Z-Zebrafish
- B-Bovine
- Pg-Pig
Product Information
Product Description
Product Usage Information
| Application | Dilution |
|---|---|
| Immunofluorescence (Immunocytochemistry) | 1:50 |
Storage
Protocol
Specificity / Sensitivity
COX IV (3E11) Rabbit mAb (Alexa Fluor® 594 Conjugate) recognizes endogenous levels of total COX IV protein.
Species Reactivity:
Human, Rat, Monkey, Zebrafish, Bovine, Pig
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys29 of human COX IV protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (1-3). It is the terminal enzyme complex in the respiratory chain, catalyzing the reduction of molecular oxygen to water coupled to the translocation of protons across the mitochondrial inner membrane to drive ATP synthesis. The 3 largest subunits forming the catalytic core are encoded by mitochondrial DNA, while the other smaller subunits, including COX IV, are nuclear-encoded. Research studies have shown that deficiency in COX activity correlates with a number of human diseases (4). The COX IV antibody can be used effectively as a mitochondrial loading control in cell-based research assays.
Limited Uses
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