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Product last modified at: 2025-01-10T19:00:12.032Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

DYRK1A (D30C10) Rabbit mAb #8765

Filter:
  • WB
  • IP
Western Blotting Image 1: DYRK1A (D30C10) Rabbit mAb
Western blot analysis of extracts from HeLa cells and mouse brain using DYRK1A (D30C10) Rabbit mAb.

To Purchase # 8765

Cat. # Size Qty. Price
8765T 20 µl
$153
8765S 100 µl
$339

Supporting Data

REACTIVITY H M R Mk
SENSITIVITY Endogenous
MW (kDa) 90
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Mk-Monkey 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

DYRK1A (D30C10) Rabbit mAb recognizes endogenous levels of total DYRK1A protein. This antibody may also recognize a background band at ~65 kDa or ~28 kDa in some cell lines.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn523 of human DYRK1A protein.

Background

The DYRK family includes several dual-specificity tyrosine-phosphorylated and regulated kinases capable of phosphorylating proteins at both Tyr and Ser/Thr residues (1). The DYRK family was identified based on homology to the yeast Yak1 (2) and the Drosophila minibrain (mnb) kinases (3). Seven mammalian isoforms have been discovered, including DYRK1A, DYRK1B, DYRK1C, DYRK2, DYRK3, DYRK4, and DYRK4B. Differences in substrate specificity, expression, and subcellular localization are seen across the DYRK family (4,5). All DYRK proteins have a Tyr-X-Tyr motif in the catalytic domain activation loop; phosphorylation of the second Tyr residue (e.g. Tyr312 of DYRK1A) is necessary for kinase activity. DYRKs typically autophosphorylate the Tyr residue within their activation loop, but phosphorylate substrates at Ser and Thr residues (1,6).
DYRK1A phosphorylates serine and threonine residues within an RPX(S/T)P consensus sequence. Substrates include transcription factors, such as FoxO1 and cAMP response element-binding proteins, such as NFAT (7,8). DYRK1A is ubiquitously expressed in fetal and adult tissues. Transgenic mice with multiple copies of DYRK1A exhibit learning and motor defects, suggesting that it is a dosage-sensitive gene (9). The DYRK1A gene localizes to chromosome 21q22.2, a region implicated by research studies in Down syndrome, and may contribute to pathological traits observed in chromosome 21 trisomy (10).
For Research Use Only. Not For Use In Diagnostic Procedures.
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