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  3. SARS-CoV-2 Spike Protein Multi-Domain (S1-NTD, RBD, S1, S2) Serological IgG ELISA Kit

SARS-CoV-2 Spike Protein Multi-Domain (S1-NTD, RBD, S1, S2) Serological IgG ELISA Kit #88005

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Inquiry Info. # 88005

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    Supporting Data

    REACTIVITY H
    Application Key:
    • ELISA-ELISA 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Description

    The SARS-CoV-2 Spike Protein Multi-Domain (S1-NTD, RBD, S1, S2) Serological IgG ELISA Kit is a solid-phase ELISA that detects binding of human IgG to four domains of SARS-CoV-2 spike protein (S-protein): the S1-NTD, RBD, S1, and S2 domains, individually. The four spike protein domains have each been individually coated onto microwells (see plate map for location/color-coding), such that twenty-four tests are provided for each spike protein domain (96 tests total). After incubation with sample, the human IgG specific for each spike protein domain is captured on the plate. The wells are then washed to remove unbound material. Anti-Human IgG, HRP-linked antibody is then used to recognize the bound IgG. HRP substrate, TMB, is added to develop color. The magnitude of optical density for this developed color is proportional to the quantity of IgG specific for each spike protein domain.

    *Antibodies in this kit are custom formulations specific to kit.

    Protocol

    Specificity / Sensitivity

    The SARS-CoV-2 Spike Protein Multi-Domain (S1-NTD, RBD, S1, S2) Serological IgG ELISA Kit detects endogenous levels of human IgG directed to the S1-NTD (16-316), RBD (318-541), S1 (16-681), and S2 (686-1208) domains of SARS-CoV-2 spike protein (S-protein).

    Species Reactivity:

    Human

    Background

    The cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is similar to other coronaviruses, and is comprised of four key structural proteins: S, the spike protein, E, the envelope protein, M, the membrane protein, and N, the nucleocapsid protein (2). Coronavirus spike proteins are class I fusion proteins and harbor an ectodomain, a transmembrane domain, and an intracellular tail (3,4). The highly glycosylated ectodomain projects from the viral envelope surface and facilitates attachment and fusion with the host cell plasma membrane. The ectodomain can be further subdivided into host receptor-binding domain (RBD) (S1) and membrane-fusion (S2) subunits, which are produced upon proteolysis by host proteases at S1/S2 and S2’ sites. S1 and S2 subunits remain associated after cleavage and assemble into crown-like homotrimers (2,4). In humans, both SARS-CoV and SARS-CoV-2 spike proteins utilize the angiotensin-converting enzyme 2 (ACE2) protein as a receptor for cellular entry (5-7). Spike protein subunits represent a key antigenic feature of coronavirus virions, and therefore represent an important target of vaccines, novel therapeutic antibodies, and small-molecule inhibitors (8,9).

    Database Links

    UniProt ID: P0DTC2


    Entrez-Gene Id: 43740568


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    For Research Use Only. Not For Use In Diagnostic Procedures.
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