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  1. Products /
  2. Cellular Assay Kits /
  3. Rapid-Act T Cell Activation Kit (Human, Anti-CD3/CD28)

Rapid-Act T Cell Activation Kit (Human, Anti-CD3/CD28) #88179

    Product Information

    Storage

    Store at 4ºC. All components in this kit are stable for 12 months when kept in the original format and stored at 4ºC. Once reconstituted, store the Anti-human CD3 and Anti-human CD28 at -80ºC. Aliquot to avoid multiple freeze/thaw cycles.

    Protocol

    Product Description

    The Rapid-Act T Cell Activation Kit (Human, Anti-CD3/CD28) provides the necessary reagents for easy, single-step induction of human T cell activation and proliferation. The included Synthetic Immune Synapse particles pre-cluster the CD3 and CD28 antibodies for thorough T cell activation, and are small enough to minimize cell clumping. Because the reagents are added to the cells in a single step, the kit enables accurate time course studies for short-term T cell stimulation. The kit can also be used for longer-term T cell proliferation and exhaustion assays. No modification or removal is necessary prior to subsequent analysis by flow cytometry.

    Specificity / Sensitivity


    Species Reactivity:

    Human

    Background

    T cells are activated by signaling through ITAM (immunoreceptor tyrosine-based activation motif)-containing CD3 signaling chains that associate with the T cell receptor (1). Co-stimulation through CD28 on T cells provides an additional signal required for effective T cell activation (2). Antibody-based binding and aggregation of CD3 and CD28 proteins simulates the engagement of a T cell with an antigen presenting cell, and results in T cell activation.

    Limited Uses

    Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
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