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Product last modified at: 2025-01-01T09:01:26.183Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

DJ-1 (D29E5) XP® Rabbit mAb (HRP Conjugate) #88652

Filter:
  • WB
Western Blotting Image 1: DJ-1 (D29E5) XP® Rabbit mAb (HRP Conjugate)
Western blot analysis of extracts from MEF, HeLa, and C6 cells using DJ-1 (D29E5) XP® Rabbit mAb (HRP Conjugate).

To Purchase # 88652

Cat. # Size Qty. Price
88652S 100 µl
$450

Supporting Data

REACTIVITY H M R Hm Mk
SENSITIVITY Endogenous
MW (kDa) 22
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Hm-Hamster 
  • Mk-Monkey 
  • Related Products

Product Information

Product Description

This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated DJ-1 (D29E5) XP® Rabbit mAb #5933.
MW (kDa) 22

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

DJ-1 (D29E5) XP® Rabbit mAb (HRP Conjugate) recognizes endogenous levels of total DJ-1 protein.

Species Reactivity:

Human, Mouse, Rat, Hamster, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys148 of human DJ-1 protein.

Background

Parkinson's disease (PD) is characterized by the presence of Lewy bodies (intracellular inclusions) and by the loss of dopaminergic neurons. Research studies have shown that mutations in α-synuclein, Parkin, and DJ-1 are linked to PD (1). α-synuclein is a major component of the aggregates found in Lewy bodies. Parkin is involved in protein degradation through the ubiquitin-proteasome pathway, and investigators have shown that mutations in Parkin cause early onset of PD (1). Loss-of-function mutations in DJ-1 cause early onset of PD, but DJ-1 is associated with multiple functions: it cooperates with Ras to increase cell transformation, it positively regulates transcription of the androgen receptor, and it may function as an indicator of oxidative stress (2-5). Dopamine D2 receptor-mediated functions are greatly impaired in DJ-1 (-/-) mice, resulting in reduced long-term depression (6).

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
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