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ProLong Gold Antifade Reagent #9071

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Immunofluorescence Image 1: ProLong  Gold Antifade Reagent
Confocal immunofluorescent analysis of HeLa cells using β-Actin (8H10D10) Mouse mAb #3700 (red) and COX IV (3E11) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4853 (green). Blue pseudocolor = DRAQ5 #4084 (fluorescent DNA dye).

To Purchase # 9071

Cat. # Size Qty. Price
9071S 10 ml
$253
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Product Information

Product Usage Information

Following immunostaining, cover samples with ProLong® Gold Antifade Reagent and coverslip.

To prevent fading, allow slides to cure in the dark, at room temperature for 24 hours, then seal the coverslips with clear lacquer, such as nail polish.

Proceed with imaging.

Storage

Store at -20°C. ProLong® Gold Antifade Reagent is stable for 12 months when stored, protected from light, at or below -20°C.

Background

ProLong Gold Antifade Reagent offers enhanced resistance to photobleaching, is premixed and ready to use, and causes little or no quenching of the fluorescent signal. The reagent cures within 24 hours and samples can be saved for months after mounting. This reagent is compatible with most dyes and is appropriate for use in multi-color labeling assays.
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    Alexa Fluor is a registered trademark of Life Technologies Corporation.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.