cdc2 (Tyr15) Control Proteins #9113
Inquiry Info. # 9113
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Supporting Data
| MW (kDa) | 32, 34 |
Product Information
Product Usage Information
As controls, CST recommends using 10 µl of GST-cdc2 negative control protein and 20 µl of the positive control SK-N-MC cell extract.
Note: Boil both negative control and positive control for 2-4 minutes prior to loading on an SDS-PAGE gel.
Storage
Product Description
Nonphosphorylated cdc2 Control Protein: A glutathione S-transferase (GST)-cdc2 fusion protein serves as a negative control for monitoring antibody phosphoselectivity for Western blotting experiments. Molecular weight of GST-cdc2 fusion protein is 32 kDa.
| MW (kDa) | 32, 34 |
Background
Description:
Phosphorylated cdc2 Control Protein: SK-N-MC cell extracts treated with hydroxyurea for 22 hours to induce cdc2 (Tyr15) phosphorylation serve as a positive control.
Nonphosphorylated cdc2 Control Protein: A glutathione S-transferase (GST)-cdc2 fusion protein serves as a negative control. The molecular weight of GST-cdc2 fusion protein is 32 kDa.
Western Blots: CST recommends using 10 µl of GST-cdc2 negative control protein and 20 µl of the positive control SK-N-MC cell extract.
Note: Boil both negative control and positive control for 2-4 minutes prior to loading on an SDS-PAGE gel.
Pathways
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Limited Uses
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