Render Target: STATIC
Render Timestamp: 2024-11-21T12:49:43.615Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-04-05 20:46:51.266
Product last modified at: 2024-11-14T17:30:09.047Z
1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Human CD3ε Activating (OKT3) Mouse mAb (Low Endotoxin, Azide-free) #92511

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Mouse IgG2a kappa
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Description

    Endotoxin Less than or equal to 0.01 EU/μg, as determined by the LaL assay.

    Formulation

    Supplied in 10 mM NaH2PO4, 150 mM NaCl, pH7.2, Low Endotoxin, Azide-free.

    Storage

    Stable for 12 months when stored at 4°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Human CD3ε Activating (OKT3) Mouse mAb (Low Endotoxin, Azide-free) recognizes endogenous levels of CD3ε protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced against human PBMC cells and purified via affinity chromatography.

    Background

    When T cells encounter antigens via the T cell receptor (TCR), information about the quantity and quality of antigens is relayed to the intracellular signal transduction machinery (1). This activation process depends mainly on CD3 (Cluster of Differentiation 3), a multiunit protein complex that directly associates with the TCR. CD3 is composed of four polypeptides: ζ, γ, ε, and δ. Each of these polypeptides contains at least one immunoreceptor tyrosine-based activation motif (ITAM) (2). Engagement of the TCR complex with foreign antigens induces tyrosine phosphorylation in the ITAM motifs and phosphorylated ITAMs function as docking sites for signaling molecules such as ZAP-70 and the p85 subunit of PI-3 kinase (3,4). TCR ligation also induces a conformational change in CD3ε, such that a proline region is exposed and then associates with the adaptor protein Nck (5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    Alexa Fluor is a registered trademark of Life Technologies Corporation.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.