Render Target: STATIC
Render Timestamp:
4/1/2025, 7:19:12 AM EDT
4/1/2025, 11:19:12 AM UTC
Commit: 461ca8d8fe5b1efd4c01fc87e5b5eb592e2d154a
XML generation date: 2025-03-07 13:14:16.997
Product last modified at: 2024-09-20T07:04:46.833Z
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PDP - Template Name: Protein Control Kit
PDP - Template ID: *******5c6809e

Bad Control Proteins #9293

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Inquiry Info. # 9293

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    Product Information

    Product Usage Information

    Boil 3 minutes prior to use. Load 10 µl of phosphorylated and nonphosphorylated Bad Control Proteins per lane.

    Storage

    Supplied in SDS Sample Buffer:
    62.5 mM Tris-HCl (pH 6.8 at 25°C),
    2% w/v SDS, 10% glycerol, 50 mM DTT,
    0.01% w/v bromophenol blue or phenol
    red. Store at –20°C.

    Product Description

    Bad Control Protein (Non-phosphorylated): Nonphosphorylated Bad peptide fusion serves as a negative control. Supplied in SDS Sample Buffer.

    Bad Control Protein (Phosphorylated): Phosphorylated Bad peptide fusion serves as a positive control. Supplied in SDS Sample Buffer.
    MW (kDa) 76

    Background

    Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL (1,2). Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136 (2). Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xL (2). Akt phosphorylates Bad at Ser136 to promote cell survival (3,4). Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK (5,6) and mitochondria-anchored PKA (7). Phosphorylation at Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL (8-10).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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