KLHL12 (2G2) Mouse mAb #9406
- WB
Supporting Data
| REACTIVITY | H M Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 62 |
| Source/Isotype | Mouse IgG1 |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
There are several lines of evidence suggesting that Kelch-like 12 protein (KLHL12) is a substrate-specific adaptor for the CUL3-based ubiquitin ligase complex. Analysis of the amino acid sequence of KLHL12 reveals an amino-terminal BTB motif, a central linker region, and a carboxy-terminal kelch domain composed of kelch repeats. Furthermore, KLHL12 has been shown to negatively regulate Wnt signaling by binding Disheveled and targeting it for ubiquitin-dependent proteasomal degradation (9). More recently, KLHL12 was shown to drive the assembly of large COPII vesicles by promoting the monoubiquitination of the COPII component Sec31. As a result, CUL3-KLHL12-dependent ubiquitination is essential for collagen export, a step that is required for integrin-dependent mouse embryonic stem cell division (10) .
- Zheng, N. et al. (2002) Nature 416, 703-9.
- Skowyra, D. et al. (1997) Cell 91, 209-19.
- Feldman, R.M. et al. (1997) Cell 91, 221-30.
- Bai, C. et al. (1996) Cell 86, 263-74.
- Singer, J.D. et al. (1999) Genes Dev 13, 2375-87.
- Winston, J.T. et al. (1999) Genes Dev 13, 2751-7.
- Kurz, T. et al. (2002) Science 295, 1294-8.
- Collins, T. et al. (2001) Mol Cell Biol 21, 3609-15.
- Angers, S. et al. (2006) Nat Cell Biol 8, 348-57.
- Jin, L. et al. (2012) Nature 482, 495-500.
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