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  1. Products /
  2. Activators & Inhibitors /
  3. Leptomycin B

Leptomycin B #9676

    Product Information

    Product Usage Information

    Leptomycin B is supplied as a 200 μM solution in ethanol within a sealed container. Please use a needle and syringe to remove the solution from the vial. All dilutions, except the final dilution, must be performed in ethanol. Final dilutions can be performed in culture media. Working concentrations and length of treatment can vary depending on the desired effect, but 1-20 nM for 3 hours generally inhibits most nuclear export. Soluble and stable in ethanol. Leptomycin B is not stable in DMSO; do not dilute in DMSO.
    In order to minimize evaporation, it is recommended that the LMB vial be kept on ice when in use.
    Stability Warning: LMB in any quantity is unstable when dried down into a film. Thus, under no circumstances should the solvent be removed from solutions of LMB because rapid decomposition and loss of recoverable material will result.

    Storage

    Store solution at -20ºC. Protect from light. If stored and handled appropriately, it will be stable for 12 months.

    Product Description

    Molecular Weight 540.73 g/mol
    Purity >98%
    Molecular Formula C33H48O6
    CAS 87081-35-4
    Solubility Soluble and stable in ethanol. Leptomycin B is not stable in DMSO; do not dilute in DMSO.

    Background

    Leptomycin B (LMB), originally discovered and utilized as a potent anti-fungal antibiotic from Streptomyces sp., has more recently been identified to inhibit nuclear export of proteins and RNA containing a Nuclear Export Sequence (NES) (1). The mechanism behind LMB's potent inhibition is achieved by specifically binding to chromosomal region maintenance (CRM)/exportin 1 protein; CRM1 binds to ribonuclear proteins containing the NES (1,2). LMB has also been reported to inhibit the degradation and subsequently lead to accumulation of p53 within the nucleus (3) and has demonstrated specific anti-tumor properties, although toxic, at high doses (1-3).

    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
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