Aurora A/B Substrate Antibody Sampler Kit #96882
Product Information
Kit Usage Information
Protocols
- 2187: Western Blotting, Immunoprecipitation (Magnetic), Immunofluorescence*
- 2914: Western Blotting, Immunofluorescence, Flow
- 3377: Western Blotting, Immunofluorescence, Flow
- 7074: Western Blotting
- 8842: Western Blotting, Immunofluorescence
- 9062: Western Blotting, Immunoprecipitation (Magnetic)
- 9713: Western Blotting, Immunofluorescence, Immunofluorescence, Flow
Product Description
Specificity / Sensitivity
Source / Purification
Background
Transforming acid coiled-coil (TACC) proteins are a family of proteins characterized by a common coiled-coil motif of approximately 200 amino acids at the carboxy-terminal end (7). When phosphorylated at Ser558 by Aurora A, mammalian TACC3 is localized to mitotic spindles and increases microtubule stability (8,9).
Aurora A-dependent phosphorylation of CENP-A on Ser7 during prophase is required for proper targeting of Aurora B to the inner centromere in prometaphase, proper kinetochore/microtubule attachment, and proper alignment of chromosomes during mitosis (10). Aurora B also targets Ser7 on CENP-A, which in turn regulates Aurora B activity during cytokinesis (11). Aurora B phosphorylates both Ser10 and Ser28 on histone H3 in concordance with mitotic chromosome condensation (12).
Aurora A phosphorylation of Thr210 on PLK promotes mitotic entry following checkpoint-dependent cell cycle arrest (13). Autophosphorylation of conserved threonine residues Thr288 (Aurora A), Thr232 (Aurora B), and Thr195 (Aurora C) is required for Aurora kinase activity (14).
- Warner, S.L. et al. (2003) Mol Cancer Ther 2, 589-95.
- Katayama, H. et al. (2003) Cancer Metastasis Rev 22, 451-64.
- Andrews, P.D. et al. (2003) Curr Opin Cell Biol 15, 672-83.
- Pascreau, G. et al. (2003) Prog Cell Cycle Res 5, 369-74.
- Crosio, C. et al. (2002) Mol Cell Biol 22, 874-85.
- Kimura, M. et al. (1999) J Biol Chem 274, 7334-40.
- Gergely, F. et al. (2000) Proc Natl Acad Sci U S A 97, 14352-7.
- Kinoshita, K. et al. (2005) J Cell Biol 170, 1047-55.
- Schneider, L. et al. (2007) J Biol Chem 282, 29273-83.
- Kunitoku, N. et al. (2003) Dev Cell 5, 853-64.
- Zeitlin, S.G. et al. (2001) J Cell Biol 155, 1147-57.
- Goto, H. et al. (2002) Genes Cells 7, 11-7.
- Macůrek, L. et al. (2008) Nature 455, 119-23.
- Willems, E. et al. (2018) Cell Div 13, 7.
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.