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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Di-Methyl-Histone H4 (Lys20) Antibody #9759

Filter:
  • WB
Western Blotting Image 1: Di-Methyl-Histone H4 (Lys20) Antibody
Western blot analysis of extracts from various cell lines using Di-Methyl-Histone H4 (Lys20) Antibody.

To Purchase # 9759

Cat. # Size Qty. Price Ships
9759S 100 µl
$357 Jan 22

Supporting Data

REACTIVITY H M R Mk
SENSITIVITY Endogenous
MW (kDa) 11
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Mk-Monkey 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Di-Methyl-Histone H4 (Lys20) Antibody detects endogenous levels of histone H4 only when di-methylated on Lys20. The antibody does not cross-react with non-, mono- or tri-methylated Lys20. In addition, the antibody does not cross-react with mono-, di- or tri-methylated histone H3 at Lys4, Lys9, Lys27 or Lys36.

Species Reactivity:

Human, Mouse, Rat, Monkey

The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

Species predicted to react based on 100% sequence homology:

D. melanogaster, Zebrafish, Bovine, Pig, C. elegans, Horse

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of histone H4 in which lysine 20 is di-methylated. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (1). Histone methylation is a major determinant for the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development (2,3). Arginine methylation of histones H3 (Arg2, 17, 26) and H4 (Arg3) promotes transcriptional activation and is mediated by a family of protein arginine methyltransferases (PRMTs), including the co-activators PRMT1 and CARM1 (PRMT4) (4). In contrast, a more diverse set of histone lysine methyltransferases has been identified, all but one of which contain a conserved catalytic SET domain originally identified in the Drosophila Su(var)3-9, Enhancer of zeste, and Trithorax proteins. Lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been implicated in both transcriptional activation and silencing (4). Methylation of these lysine residues coordinates the recruitment of chromatin modifying enzymes containing methyl-lysine binding modules such as chromodomains (HP1, PRC1), PHD fingers (BPTF, ING2), tudor domains (53BP1), and WD-40 domains (WDR5) (5-8). The discovery of histone demethylases, such as PADI4, LSD1, JMJD1, JMJD2, and JHDM1, has shown that methylation is a reversible epigenetic marker (9).

Pathways

Explore pathways related to this product.


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