Cleaved Caspase-8 (Asp387) (D5B2) XP® Rabbit mAb (BSA and Azide Free) #97756
- WB
- IF
- F
Supporting Data
REACTIVITY | M |
SENSITIVITY | Endogenous |
MW (kDa) | 18, 43 |
Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Usage Information
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
Formulation
Storage
Specificity / Sensitivity
Cleaved Caspase-8 (Asp387) (D5B2) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of mouse caspase-8 only when cleaved at Asp387. In addition to the p18 subunit, this antibody also detects cleavage products containing the pro-domain with the p18 subunit (p43).
Species Reactivity:
Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp387 of mouse caspase-8 protein.
Background
Apoptosis induced through the CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10 (1-3). Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6, and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage.
Limited Uses
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