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PDP - Template Name: Antibody Sampler Kit
PDP - Template ID: *******4a3ef3a

Rb Antibody Sampler Kit #9969

Rb Antibody Sampler Kit: Image 1
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

To Purchase # 9969

Cat. #

Size

9969T
1 Kit
  • Product Includes
  • Related Products
Product IncludesQuantityApplicationsReactivityMW(kDa)Isotype
Phospho-Rb (Ser780) (D59B7) Rabbit mAb #818020 µlWB, IPH M R Mk110Rabbit IgG
Phospho-Rb (Ser795) Antibody #930120 µlWB, IPH Mk110Rabbit 
Phospho-Rb (Ser807/811) (D20B12) XP® Rabbit mAb #851620 µlWB, IP, IHC, IF, FH M R Mk110Rabbit IgG
Rb (4H1) Mouse mAb #930920 µlWB, IP, IHC, IF, F, ChIPH Mk B Pg110Mouse IgG2a
Anti-rabbit IgG, HRP-linked Antibody #7074100 µlWBRabGoat 
Anti-mouse IgG, HRP-linked Antibody #7076100 µlWBMHorse 

Product Information

Product Description

The Rb Antibody Sampler Kit provides reagents and protocols to investigate cell cycle progression within cells. The kit contains primary and secondary antibodies to perform two Western blot experiments with each antibody.

Specificity / Sensitivity

All antibodies contained in this kit detect endogenous levels of their respective target protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide and are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with recombinant human proteins or synthetic peptides.

Background

The retinoblastoma tumor suppressor protein Rb regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9).

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
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