Chloroquine #14774
Product Information
Product Usage Information
Chloroquine is supplied as a lyophilized powder. For a 50 mM stock, reconstitute the 150 mg in 5.82 ml sterile dH2O. First add 1 ml dH2O to the tube containing the chemical, vortex, and dispense into a new, larger tube. Repeat this action two or three more times to transfer any residual material. Add additional dH2O to the new tube to bring the volume up to 5.82 ml. Filter sterilize into sterile tube. Utilize a syringe and 0.2 μm syringe filter to minimize sample loss.
Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 25-100 μM for 12-48 hr.
Storage
Product Description
| Molecular Weight | 515.9 g/mol |
| Purity | >98% |
| Molecular Formula | C18H26ClN3•2H3PO4 |
| CAS | 50-63-5 |
| Solubility | Soluble in H2O at 25mg/ml. |
Background
Chloroquine treatment of cells leads to accumulation of light chain 3-II (LC3-II) (1-3). This autophagy marker resides within autophagosomal membranes during the autophagic process and is degraded upon fusion with lysosomes. Chloroquine inhibition of these fusion events effectively blocks LC3-II degradation.
This product has applications to SARS-CoV-2 research into the mechanisms of the Novel Coronavirus, which has caused the COVID-19 pandemic.
Limited Uses
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